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Candidate Drug Predicted Using DSigDB

Journal: Journal of Inflammation Research

Article Title: Identifying Therapeutic Targets and Potential Drugs for Diabetic Retinopathy: Focus on Oxidative Stress and Immune Infiltration

doi: 10.2147/JIR.S500214

Figure Lengend Snippet: Candidate Drug Predicted Using DSigDB

Article Snippet: Primary antibodies targeting CCL4 (1:1000, Bioss, bs-2475R, China), FCGR2B (1:1000, Abclonal, A12553, China) and β-actin (1:1000, ZSGB-BIO, TA-09, China) overnight at 4°C.

Techniques:

Docking Results of Available Proteins With Candidate Drugs

Journal: Journal of Inflammation Research

Article Title: Identifying Therapeutic Targets and Potential Drugs for Diabetic Retinopathy: Focus on Oxidative Stress and Immune Infiltration

doi: 10.2147/JIR.S500214

Figure Lengend Snippet: Docking Results of Available Proteins With Candidate Drugs

Article Snippet: Primary antibodies targeting CCL4 (1:1000, Bioss, bs-2475R, China), FCGR2B (1:1000, Abclonal, A12553, China) and β-actin (1:1000, ZSGB-BIO, TA-09, China) overnight at 4°C.

Techniques: Binding Assay

Molecular docking simulation diagram. ( A ) (+)-chelidonine binds to CCL4. ( B ) oxazolone binds to CCL4. ( C ) eugenol binds to CCL4. ( D ) simvastatin binds to CCL4. ( E ) (+)-chelidonine binds to FCGR2B. ( F ) oxazolone binds to FCGR2B. ( G ) eugenol binds to FCGR2B. ( H ) AGN-PC-0JHFVD binds to FCGR2B. ( I ) AGN-PC-0JHFVD binds to FOXP3. ( J ) simvastatin binds to FOXP3. CCL4 is marked in red, FCGR2B in wheat, FOXP3 in green, and candidate drugs in cyan. Blue solid lines represent hydrogen bonds, grey dotted lines represent hydrophobic interactions, and green dotted lines represent π-stacking interactions.

Journal: Journal of Inflammation Research

Article Title: Identifying Therapeutic Targets and Potential Drugs for Diabetic Retinopathy: Focus on Oxidative Stress and Immune Infiltration

doi: 10.2147/JIR.S500214

Figure Lengend Snippet: Molecular docking simulation diagram. ( A ) (+)-chelidonine binds to CCL4. ( B ) oxazolone binds to CCL4. ( C ) eugenol binds to CCL4. ( D ) simvastatin binds to CCL4. ( E ) (+)-chelidonine binds to FCGR2B. ( F ) oxazolone binds to FCGR2B. ( G ) eugenol binds to FCGR2B. ( H ) AGN-PC-0JHFVD binds to FCGR2B. ( I ) AGN-PC-0JHFVD binds to FOXP3. ( J ) simvastatin binds to FOXP3. CCL4 is marked in red, FCGR2B in wheat, FOXP3 in green, and candidate drugs in cyan. Blue solid lines represent hydrogen bonds, grey dotted lines represent hydrophobic interactions, and green dotted lines represent π-stacking interactions.

Article Snippet: Primary antibodies targeting CCL4 (1:1000, Bioss, bs-2475R, China), FCGR2B (1:1000, Abclonal, A12553, China) and β-actin (1:1000, ZSGB-BIO, TA-09, China) overnight at 4°C.

Techniques:

External validation of CCL4 and FCGR2B. ( A ) The mRNA levels of CCL4 and FCGR2B were evaluated in animal models by RT-qPCR. ( B ) The protein levels of CCL4 and FCGR2B were evaluated animal models by WB. ** represents P <0.01, * represents P <0.05 and ns, no significance.

Journal: Journal of Inflammation Research

Article Title: Identifying Therapeutic Targets and Potential Drugs for Diabetic Retinopathy: Focus on Oxidative Stress and Immune Infiltration

doi: 10.2147/JIR.S500214

Figure Lengend Snippet: External validation of CCL4 and FCGR2B. ( A ) The mRNA levels of CCL4 and FCGR2B were evaluated in animal models by RT-qPCR. ( B ) The protein levels of CCL4 and FCGR2B were evaluated animal models by WB. ** represents P <0.01, * represents P <0.05 and ns, no significance.

Article Snippet: Primary antibodies targeting CCL4 (1:1000, Bioss, bs-2475R, China), FCGR2B (1:1000, Abclonal, A12553, China) and β-actin (1:1000, ZSGB-BIO, TA-09, China) overnight at 4°C.

Techniques: Quantitative RT-PCR